Everyclick’s online corporate sponsorship package to benefit charities

first_img AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis Polly Gowers, CEO of everyclick.com said: “Everyclick.com was designed to create a new, sustainable and free source of income for charities. Our new sponsorship package fulfills that brief. It gives charities access to unrestricted income and information about their supporters and companies a unique platform to enhance their brand.”This is one further option to present to a charity’s existing corporate supporters. If they are going to advertise on a search engine they should consider everyclick given the financial benefit to charity. Tagged with: corporate Digital  24 total views,  1 views today AddThis Sharing ButtonsShare to TwitterTwitterShare to FacebookFacebookShare to LinkedInLinkedInShare to EmailEmailShare to WhatsAppWhatsAppShare to MessengerMessengerShare to MoreAddThis Howard Lake | 1 March 2007 | Newscenter_img Everyclick’s online corporate sponsorship package to benefit charities Everyclick.com, the search engine that helps charities, has created a new sponsorship package that will help companies promote their brand and corporate social responsibility (CSR) credentials on the site, while at the same time generating income for the company’s designated charity.Everyclick’s is offering three levels of sponsorship . In each case, Everyclick splits the advertiser’s advertising expenditure with a charity, which the company can choose.Companies can choose sponsorship over the entire site, on a particular search category, or on a seasonal campaign basis. Each package includes a data capture option. Data can be shared with the charity or the company. Advertisement About Howard Lake Howard Lake is a digital fundraising entrepreneur. Publisher of UK Fundraising, the world’s first web resource for professional fundraisers, since 1994. Trainer and consultant in digital fundraising. Founder of Fundraising Camp and co-founder of GoodJobs.org.uk. Researching massive growth in giving.last_img read more

A Taste of Food, Wine and Duarte

first_imgChambers of Commerce News A Taste of Food, Wine and Duarte The 17th annual Taste of Duarte and Business Expo is serving up another fun night of food, music and drinks! By FRANZ A.D. MORALES Published on Monday, April 29, 2013 | 1:44 pm 10 recommended0 commentsShareShareTweetSharePin it Indulge your senses and sharpen your business acumen this May 8 as the Duarte Chamber of Commerce hosts its 17th annual Taste of Duarte and Business Expo.Over 20 restaurants and 40 businesses will be participating this year, in what will surely be another fun-filled night of food, drink, music and more.“We have a live band and it’s kind of an outdoor festival sale because it’s on grass and in a beautiful courtyard,” says Diane Burckhard, Marketing Director for the Duarte Chamber of Commerce.Free food and drink samples will be available courtesy of the participating restaurants, which include: Matt Denny’s Ale house, Sushiya, Flame Broiler, California Skewers and many more.But more than the food tasting, being a business expo, there will also be “quite a few home-based businesses. It’s an opportunity for them to market their business. We have banks and different types of businesses; martial arts, computer business, and the YMCA of course, for the kids,” says Burckhard.Burckhard explains that the business expo will showcase Duarte’s many networking opportunities, as well as other Duarte business-related programs.“We have DC Bankers with breakfast mixers, mixed with other chambers in the neighboring communities, and we have workshops on how to expand your business, market your business; free workshops,” says Burckhard.The band Cold Duck, an 8-piece band led by Jose A. Cuadra and arguably the best dance band in Southern California today, will be providing the night’s music and entertainment, together with raffles, wine and food tasting, and a small car show of the latest models.The event will be held at the Westminster Gardens at 1420 Santo Domingo, Duarte and tickets are as follows: $35 for adults and children above 13 years old $25 for seniors aged 65+ and children from aged 6-12 Children under 6 enter for free To purchase tickets and to find out more about the event, visit http://www.tasteofduarte.com or call (626) 357-3333. You can also email: [email protected] To learn more about Cold Duck, visit http://www.coldduck.com. Business News Your email address will not be published. Required fields are marked * Subscribe Community News Name (required)  Mail (required) (not be published)  Website  Make a comment faithfernandez More » ShareTweetShare on Google+Pin on PinterestSend with WhatsApp,Virtual Schools PasadenaHomes Solve Community/Gov/Pub SafetyPASADENA EVENTS & ACTIVITIES CALENDARClick here for Movie Showtimes Top of the News center_img EVENTS & ENTERTAINMENT | FOOD & DRINK | THE ARTS | REAL ESTATE | HOME & GARDEN | WELLNESS | SOCIAL SCENE | GETAWAYS | PARENTS & KIDS First Heatwave Expected Next Week More Cool Stuff Pasadena Will Allow Vaccinated People to Go Without Masks in Most Settings Starting on Tuesday Get our daily Pasadena newspaper in your email box. Free.Get all the latest Pasadena news, more than 10 fresh stories daily, 7 days a week at 7 a.m. HerbeautyBohemian Summer: How To Wear The Boho Trend RightHerbeautyHerbeautyHerbeauty15 Countries Where Men Have Difficulties Finding A WifeHerbeautyHerbeautyHerbeautyIs It Bad To Give Your Boyfriend An Ultimatum?HerbeautyHerbeautyHerbeautyNutritional Strategies To Ease AnxietyHerbeautyHerbeautyHerbeautyGained Back All The Weight You Lost?HerbeautyHerbeautyHerbeauty9 Of The Best Family Friendly Dog BreedsHerbeautyHerbeauty Community News Pasadena’s ‘626 Day’ Aims to Celebrate City, Boost Local Economy Home of the Week: Unique Pasadena Home Located on Madeline Drive, Pasadenalast_img read more

[Breaking] Law Student Files Contempt Petition Against Kunal Kamra Over His Tweets On Supreme Court With Sanction From Attorney General

first_imgTop Stories[Breaking] Law Student Files Contempt Petition Against Kunal Kamra Over His Tweets On Supreme Court With Sanction From Attorney General LIVELAW NEWS NETWORK13 Nov 2020 4:04 AMShare This – x”The contemnor stands in scarce regard of law, his intention to demean, scandalize and assault the judicial institution is evident from the series of his conduct”, Says the petition.Skand Bajpai, a fourth-year law student at New Law College, Bharati Vidyapeeth Deemed to be University has moved Supreme Court seeking to initiate criminal contempt of Court proceedings against comedian Kunal Kamra, after he obtained the required sanction from Attorney General KK Venugopal.He has approached the Supreme Court under Article 129 of the Constitution of India read with Section 15…Your free access to Live Law has expiredTo read the article, get a premium account.Your Subscription Supports Independent JournalismSubscription starts from ₹ 599+GST (For 6 Months)View PlansPremium account gives you:Unlimited access to Live Law Archives, Weekly/Monthly Digest, Exclusive Notifications, Comments.Reading experience of Ad Free Version, Petition Copies, Judgement/Order Copies.Subscribe NowAlready a subscriber?LoginSkand Bajpai, a fourth-year law student at New Law College, Bharati Vidyapeeth Deemed to be University has moved Supreme Court seeking to initiate criminal contempt of Court proceedings against comedian Kunal Kamra, after he obtained the required sanction from Attorney General KK Venugopal.He has approached the Supreme Court under Article 129 of the Constitution of India read with Section 15 of the Contempt of Courts Act, 1975 and Rule 3(c) of the Rules to Regulate Proceedings for Contempt of The Supreme Court, 1975Kamra had made a series of tweets regarding the Supreme Court and initiation of contempt against him has been sought for 4 of his tweets posted in the wake of the Supreme Court granting interim bail to Arnab Goswami.Complaining against the same Bajpai had sent a letter to the AG stating that the tweets and publications scandalize the Supreme Court and prejudices the minds of millions of people.The Attorney General for India, KK Venugopal has granted the petitioner his consent for initiation of contempt proceedings against comedian Kunal Kamra, over his tweets about the Supreme Court in the wake of its order granting interim bail to Arnab Goswami.The petitioner has submitted that he, after receiving consent from Ld. The Attorney General of India wrote to the contemnor on his email address “[email protected]” with bona fide motives informing him of the letter of consent to initiate criminal contempt of court and humbly requesting the contemnor to delete all his tweets dated 11.11.2020 as mentioned in the petitioner’s address to the Ld. Attorney General for India and further requested him to tender an unconditional apology on all his social media handles for his malicious, scandalous, and contemptuous deeds within 6 hours of the email by the petitioner, failing which shall compel the petitioner to initiate criminal contempt of court proceedings against the contemnor.”That the contemnor on 13.12.2020, has made it clear that he would not retract his tweets dated 11.11.2020 and further went on to say that the statue of Mahatma Gandhi in the Court premises be replaced with that of Sr. Advocate Mr. Harish Salve and the portrait of Late Pt. Jawaharlal Nehru be replaced with that of Sr. Advocate Mr. Mahesh Jethmalani”.The petitioner alleged that  it is evident from the above posts and tweets that the contemnor was knowingly insulting the institution, each post/tweet has been made with the intention to shake the trust of general public from the apex court.”The contemnor stands in scarce regard of law, his intention to demean, scandalize and assault the judicial institution is evident from the series of his conduct. The contemnor even after several cautions proceeded further with a series of contemptuous tweets and posts and has wilfully ignored the request of the petitioner to delete the contemptuous content and tender an unconditional apology”.The petitioner has also submitted that the tweets  and publication thereof scandalises the Supreme Court, prejudices and interferes with the due course of judicial proceedings and administration of justice. Such comments scandalizing and lowering the authority of the Court in relation to the judicial matters but also have the effect of substantial interference with obstructing the administration of justice. Unfounded and unwarranted aspersions have the tendency to undermine the authority of the Court and would create distrust in the mind of the public and on the capacity to impart fearless justice.Subscribe to LiveLaw, enjoy Ad free version and other unlimited features, just INR 599 Click here to Subscribe. All payment options available.loading….Next Storylast_img read more

Calls for spending Ballyliffen’s Renewal Scheme allocation ahead of Irish Open

first_img Google+ By News Highland – November 1, 2017 Homepage BannerNews Twitter WhatsApp WhatsApp Pinterest DL Debate – 24/05/21 Facebook News, Sport and Obituaries on Monday May 24th Journey home will be easier – Paul Hegarty Pinterestcenter_img Google+ Arranmore progress and potential flagged as population grows Calls for spending Ballyliffen’s Renewal Scheme allocation ahead of Irish Open Facebook Previous articleMan’s body recovered from River FoyleNext articleTalks aimed at restoring power sharing at Stormont resume this morning News Highland Harps come back to win in Waterford RELATED ARTICLESMORE FROM AUTHOR Important message for people attending LUH’s INR clinic An Inishowen Councillor is calling for money allocated to Ballyliffen under the Town and Village Renewal Scheme to be spent ahead of the Irish Open next year.The main aim of the scheme is to support the revitalisation of towns and villages in order to increase their potential to support increased economic activity into the future.Ballyliffen was awarded 100,000 in the latest round of funding.Cllr Martin McDermott says it’s important for the village to look its best before May, therefore the Council need to press ahead with spending the money:Audio Playerhttp://www.highlandradio.com/wp-content/uploads/2017/11/martinmddfgfdgd.mp300:0000:0000:00Use Up/Down Arrow keys to increase or decrease volume. Twitterlast_img read more

More PPE arrives in NI as Covid-19 case numbers rise

first_img Important message for people attending LUH’s INR clinic WhatsApp Pinterest There have now been 244 deaths on the island of Ireland from Covid-19, 174 in the Republic and 70 in the North.The cumulative number of confirmed cases is now over 6,500, 103 in Donegal and 51 in the Derry City and Strabane district.A large amount of personal protective equipment arrived in the North yesterday.Deputy first minister Michelle O’Neill says this will address a significant shortage of the gear for healthcare workers……………Audio Playerhttps://www.highlandradio.com/wp-content/uploads/2020/04/1106oneill.mp300:0000:0000:00Use Up/Down Arrow keys to increase or decrease volume. Twitter Previous articleRestrictions likely to be extended beyond EasterNext articleÚdaras na Gaeltachta seeking mentors for enterprise programme News Highland More PPE arrives in NI as Covid-19 case numbers rise WhatsApp By News Highland – April 7, 2020 Facebook Pinterest Google+center_img Facebook Twitter AudioHomepage BannerNews DL Debate – 24/05/21 RELATED ARTICLESMORE FROM AUTHOR Arranmore progress and potential flagged as population grows Derry draw with Pats: Higgins & Thomson Reaction FT Report: Derry City 2 St Pats 2 Google+ News, Sport and Obituaries on Monday May 24th last_img read more

NBA player Chandler Parsons faces potentially career-ending injury after car hit by drunk driver, law firm says

first_img Beau Lund January 20, 2020 /Sports News – National NBA player Chandler Parsons faces potentially career-ending injury after car hit by drunk driver, law firm says FacebookTwitterLinkedInEmailiStock(NEW YORK) — NBA player Chandler Parsons was in a potentially career-ending car accident, reportedly involving a driver who was under the influence, according to the law firm Morgan & Morgan.Chandler, who is a small forward with the Atlanta Hawks, was reportedly driving home from practice around 2 p.m. on Jan. 15 when his vehicle was struck. Parsons, according to his attorneys, “suffered multiple severe and permanent injuries including a traumatic brain injury, disc herniation and a torn labrum.”Parsons’ attorneys, John Morgan and Nick Panagakis, said his injuries could potentially end his nine-year NBA career.“Chandler is having a difficult time accepting the consequence of the defendant’s reckless conduct on the roadway,” his lawyers said in a statement to ABC News.The alleged suspect was not identified by the firm, but it said the driver was arrested and charged with a DUI. The driver who allegedly hit Parsons was unconscious at the wheel when police arrived, according to USA Today, citing an incident report. A 7-Up bottle filled with alcohol was reportedly found in the driver’s seat, the outlet reported.The Hawks, in a statement on the day of the incident, said Parsons was involved in an accident after practice. The team said he was diagnosed with a concussion and whiplash and that he would enter the NBA’s concussion protocol. They announced Monday he was out of their next game with “concussion/whiplash/associated disc injury.”The team has not disclosed any further details about his injuries or the crash.“Chandler was in peak physical condition at the time of the wreck,” Parsons’ legal team said in a statement. “He is now working with a team of doctors to regain his health, and at this time, his ability to return to play is unclear. Our focus right now is on helping him make a full recovery, while we also work to hold any and all responsible parties fully accountable.”Parsons is on the last year of a four-year, $94 million contract. He was traded from the Memphis Grizzlies to the Hawks prior to the start of the 2019-2020 NBA season.The forward was selected by the Houston Rockets in the first round of the 2011 NBA Draft. He has also played for the Dallas Mavericks. In college, Parsons was named an NCAA All-American while playing for the Florida Gators. Copyright © 2020, ABC Audio. All rights reserved.center_img Written bylast_img read more

Another senior Purplebricks exec leaves to join hybrid competitor

first_imgThe senior brain drain from Purplebricks continues apace after the company’s Lettings Operations Director Howard Russell yesterday revealed that he has left to join a competitor.30-year-old Russell was at Purplebricks for five years and, like many of his contemporaries, joined as an LPE after an early career in independent lettings at a Birmingham high street agency.He was then promoted through the ranks to establish its lettings operation but has now decided to join Accommodation.co.uk, the recently-launched hybrid lettings platform that is in direct competition with his former employer.“I had an amazing time at Purplebricks because everyone there had a passion for the business, but it is going through a significant transition at the moment under its new leadership and there’s been a cultural shift that hasn’t suited everyone,” he told The Negotiator.Based at its headquarters in Manchester, Russell’s new job is to manage Accommodation’s growing team of Local Letting Consultants (LLCs), many of whom also have Purplebricks backgrounds.“I joined because they share my vision of what the future of estate agency looks like,” he says.“There are a lot of lettings technologies out there at the moment but there isn’t one that is able to bring it all together and produce an end-to-end digital journey for a landlord.”Russell says he watched Purplebricks grow from its early days and says it’s that experience he’s bringing to Accommodation.co,uk, which is only five weeks old.“You’ve got to be able to turn your hand to most things and have a good spread of abilities during the start-up phase,” he says.Accommodation so far has LLCs in Manchester, Leeds, Telford, Swansea, Lincoln and Stockport and this month will open operations in Liverpool and Canterbury.  Howard Russell Accommodation Purplebricks November 5, 2019Nigel LewisWhat’s your opinion? Cancel replyYou must be logged in to post a comment.Please note: This is a site for professional discussion. Comments will carry your full name and company.This site uses Akismet to reduce spam. Learn how your comment data is processed.Related articles Letting agent fined £11,500 over unlicenced rent-to-rent HMO3rd May 2021 BREAKING: Evictions paperwork must now include ‘breathing space’ scheme details30th April 2021 City dwellers most satisfied with where they live30th April 2021 Home » News » Agencies & People » Another senior Purplebricks exec leaves to join hybrid competitor previous nextAgencies & PeopleAnother senior Purplebricks exec leaves to join hybrid competitorHoward Russell says he’s joining Accommodation.co.uk partly because the company culture at Purplebricks has changed dramatically after the Bruce brothers left.Nigel Lewis5th November 201902,004 Viewslast_img read more

News story: £800 million agreed for defence

first_imgThis includes access to £600 million from the Dreadnought contingency, announced in 2015, and will ensure that the UK’s new world-class nuclear submarines are delivered on time and within the £31 billion budget agreed at the Strategic Defence and Security Review in 2015.In addition £200 million was agreed at the Supplementary Estimates earlier in the year.A Royal Navy submarine has always been on patrol, providing the continuous at sea deterrent for almost 50 years. And the next generation of submarines will ensure this can continue into the future, deterring conflict and protecting the nation 24 hours a day, 7 days a week, 365 days a year.This money funds a key government priority that has been the bedrock of defence policy for nearly 50 years.Chancellor of the Exchequer, Philip Hammond, said: Defence Secretary Gavin Williamson said: Our commitment to defence and national security is unwavering. The UK’s defence budget is the highest in Europe and the second highest in NATO and ensures Britain can continue to respond effectively to the ever-changing threats we face. We will continue to invest in our world-class Armed Forces and this additional investment of £600 million will ensure the UK is protected by the nuclear deterrent provided by the new Dreadnought fleet into the 2030s and beyond.center_img This is a welcome boost to our Armed Forces, ensuring we can continue to back-up our national security with the ultimate capability. In a world of intensifying and evolving threats, we must strengthen and maintain our ability to seize opportunities and counter challenges as they emerge. I have launched the Modernising Defence Programme to ensure we have the capabilities we need to keep Britain safe in an increasingly dangerous world.last_img read more

Zines were the scene

first_imgZines, self-published, self-distributed works tackling topics overlooked by the mainstream media, were a popular form of expression and communication in the 1980s and early ’90s. They were quite often described as labors of love by their authors.Over the summer, they became a labor of love for two Harvard seniors. Caitlin Ballotta and Nora Garry spent 10 weeks in Widener Library poring over the recent acquisition of about 20,000 zines and related materials. It’s been an eye-opening experience for the two women, and an important look at the issues and the stories that dominated the fringes of pop culture before the arrival of the Internet.Garry, as a concentrator in studies of women, gender, and sexuality, has had some exposure to zines, as gender was a major topic tackled by the publications.“For me, I will pick up a zine and see that it’s written by someone we have read about in class. So while I knew a little bit about zines, here I have the chance to actually go beyond just reading about them,” she said.Ballotta, an English concentrator, had little to no exposure to zines prior to beginning her internship. But she quickly realized the importance of ensuring that the zines are available to scholars.“There is a very big difference between working with source material versus reading someone else’s description of the material,” she said. “I find zines very interesting because they are hybrid publications. They don’t really fit into any one genre. And they are intensely personal, but they are intended to be publicized.”Ballotta and Garry came to Widener as part of the Summer Humanities and Arts Research Program (SHARP). This summer marked the pilot of the 10-week immersion program designed to give undergraduates opportunities to engage in substantive humanities- and arts-based research. SHARP, funded in part by the Dean’s Leadership Fund, joins other established summer research programs for undergraduates, including the Program for Research in Science and Engineering (PRISE), Behavioral Laboratory in the Social Sciences (BLISS), and the Program for Research in Markets & Organizations (PRIMO). The students in the programs lived and ate together throughout the 10 weeks, sharing their experiences.“This first year of the program has just been absolutely fantastic, and connecting them with PRIMO, PRISE, and BLISS really created this wonderful intellectual community,” said Diana Sorensen, dean of the arts and humanities. “We have been searching for funding for a few years to get the SHARP program up and running. And thanks to Dean [Michael D.] Smith, we were able to make this possible. Having spent time with the students this summer, it was tremendous to see these brilliant people coming together around their intellectual passions.”Nine students participated in the SHARP projects.“Before this program launched, there weren’t many research opportunities in the humanities, so this is a great thing for us,” Ballotta said.Garry and Ballotta are interested in becoming librarians, which prompted them to apply for the zines project. It has allowed them to meet with library staff — from collections developers to research librarians to catalogers — so that the two received an in-depth view of the libraries and how they support the curriculum.As for the zines, the seniors said working with the material has been a tremendous experience. Zines became a voice for the counterculture, exploring genres of art, literature, and politics, further broken down into subgenres including punk rock, gender, and sexuality.  A zine culture developed as authors, known as “zinesters,” developed a network to distribute their homegrown material. In the days before Web pages, blogs, Facebook, and Twitter, zinesters made their voices heard by toiling over typewriters, photocopying pages, and compiling them into zines to be mailed to anyone who wanted to read them.The intention of a zine was not to make money, but to make a point.“Oftentimes, these were money losers,” Garry said. “These zines are important because they not only capture a particular time, but they capture a particular mindset from a particular point of view.”With the dawn of the digital age, zinesters moved to blogs and social networks.“The Internet created broad access. People could have such a far broader reach if they published on the Internet rather than mail out their zines. But with that, something was lost,” Ballotta said. “You lost the ‘materiality.’ There was no template for zines, but when you publish a blog or a website, there is a template, so there isn’t that originality. There was also a culture created around how zines were distributed, which again is lost when you go digital.”After weeks of working with a major collection of primary source materials, Garry and Ballotta created a project titled “Enlivening Zines for Study and Research.”They said the experience has also been enriching in ways that went beyond the zines.  Although the two didn’t know each other before the program, they got to know each other very well, bonding over the zines.“For my birthday,” Garry said, “Caitlin made me a zine.”last_img read more

Epidemic of autoimmune diseases calls for action

first_img Researchers develop ‘GPS’ method to guide mesenchymal stem cells to inflammatory sites Related The immune system normally defends the body against everything from microscopic viruses to meters-long parasitic worms. But sometimes, it sets its sights on the wrong target: the body’s own cells.Autoimmunity problems cause many different diseases, depending on which cells are targeted. In Type 1 diabetes, it’s beta cells. Beta cells are located in the pancreas and produce insulin, the molecule necessary to convert food into energy. Without it, patients must monitor their blood and inject themselves with insulin multiple times a day.Scientists at the Harvard Stem Cell Institute (HSCI) want to develop a better treatment: transplanting healthy beta cells grown in the laboratory into patients. Having figured out the first part of the process, they are now figuring out how to protect newly transplanted cells from autoimmune attack.Autoimmune epidemicArlene Sharpe is the George Fabyan Professor of Comparative Pathology at Harvard Medical School. She said that over the past 30 years, autoimmune diseases have become almost epidemic.“They’re increasing in frequency and occurring earlier in life, and we don’t understand why,” she said. “There’s tremendous need to understand the causes of these diseases and how to treat them.”Sharpe said that in the case of Type 1 diabetes, using a patient’s own stem cells — which have the ability to become many types of cells — is a promising therapy. By coaxing the stem cells to become beta cells in the lab, researchers can give patients their own source of insulin.“One of the challenges now is to be able to deliver these cells in such a way that the body tolerates them,” Sharpe said. “It’s leading to a whole new field of immunology: regenerative immunology.”Protecting the beta cellHSCI co-director Douglas Melton, who discovered how to make beta cells from stem cells in 2014, is now turning his attention to the immunity problem. He said that scientists still do not understand exactly how the immune system attacks beta cells.“We’ve decided to protect the beta cell without knowing all the different ways that the immune system is trying to kill it. We want to genetically modify the beta cell to make it invisible to the immune system,” said Melton, who is the Xander University Professor in the Harvard Department of Stem Cell and Regenerative Biology. Over the past 30 years, autoimmune diseases have become almost epidemic. — Arlene Sharpe, Harvard Medical School One of Melton’s approaches is to take molecules that are already known to protect cells from the immune system in certain situations, and add them to the beta cells. Pregnancy is a familiar example. A pregnant woman’s immune system does not attack the fetus, even though half of the fetus’s genes come from the father and are foreign to her. The fetus survives because it expresses protective molecules. Cancer cells survive for a similar reason. They express molecules that allow them to evade detection by the immune system.Searching for new genesThe Melton lab is also searching for entirely new ways of protecting beta cells, using an experiment called a genetic screen.“When we grow cells to transplant them into a person, we grow a lot of them: around 500 million,” Melton said. “We’ve removed one gene at a time from each cell. Since there are only 20,000 genes, we could do that easily with 500 million cells.”Most of the mutated cells would be killed by the patient’s immune system.“But wouldn’t it be fantastic if there was some mutation that said to the immune system, ‘You can’t see me — leave me alone!’” he said.Testing the genesNayara Leite is a postdoctoral researcher in Melton’s lab. She is developing a method to test whether any of those genetic modifications protect beta cells from immune attack.Leite uses blood donated by patients with Type 1 diabetes for two purposes: isolating immune cells, and making stem-cell-derived beta cells.,By putting these donor-matched cells together in a petri dish, she expects the immune cells to react and kill the beta cells — unless the beta cells contain a protective genetic modification.“For Type 1 diabetes, the main autoreactive immune cells will be close to the pancreas, so the frequency of these cells is very low in the blood,” Leite said. “In the beginning it was very hard to get a reaction, because you almost didn’t have anything there.”Leite is continuing to refine the method. She is also starting to genetically modify patient beta cells, focusing on genes identified by Stephan Kissler and Peng Yi, who collaborate with the Melton lab.A shot in the darkKissler is a Harvard assistant professor based at Joslin Diabetes Center. He and Yi — both of whom are HSCI affiliate faculty — have completed a genetic screen similar to the one Melton is planning, using a mouse experimental system.“We took an approach that was a bit of a shot in the dark,” Kissler said. The researchers mutated beta cells, ending up with a mixture of millions of cells that carried different mutations in every possible gene.“We put the cells in a diabetic mouse, and then we just waited,” Kissler said. “The immune system killed off beta cells like it normally does. After two months, we went back and found a small number of cells that were still there.”The protected beta cells had mutations in a dozen different genes. In follow-up mouse experiments that directly compared regular and modified beta cells, most of the gene mutations panned out.“Regular beta cells that aren’t protected with the mutation are gone within days, and beta cells with the mutation kick around for weeks,” Kissler said.Next stepsKissler said that none of the protective genes he and Yi identified were obvious choices, so they are investigating the mechanisms of how these genes are involved in autoimmunity. Kissler and Yi are also collaborating with the Melton lab to test the genetic modifications in a human cell experimental system.“Our two top candidate genes are associated with diabetes in people, and we’re very excited because that provides relevance to human disease,” Kissler said. “That gives us a lot of hope that if we were to do this in human cells, we might get similar effects.”Beyond just protecting beta cells, Kissler and Yi want to understand more about Type 1 diabetes and why autoimmunity occurs.“Maybe we can learn from our experiments more about the disease itself — maybe we can work our way back to how does it all start in the first place,” Kissler said. Role of gut bacteria in averting Type 1 diabetes Study reveals how specific gene and intestinal bacteria work together Steering stem cell trafficking into pancreas reverses Type 1 diabeteslast_img read more